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If building v2 sample sheets for instruments apart from the ones shown, affirm the ultimate sample sheet has the i5 index in the proper orientation as explained above.
Index 1: the i7 index column is while in the ahead orientation (match the orientation if the i7 indexes within the sample sheet).
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Established A Index Adapters Consult with Index 2 (i5) Orientation For more info regarding how to enter i5 bases over the sample sheet in forward or reverse enhance orientation.
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This permits substantial figures of here libraries to be pooled and sequenced at the same time for the duration of just one operate. When compared to other indexing approaches, exclusive dual indexing mitigates index hopping by filtering hopped reads.
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NextSeq a thousand/2000 and NovaSeq X/X Moreover i5 indexes will the right way be output while in the forward orientation for later on downstream processing. Customized kits also needs to possess the i5 indexes entered during the forward orientation.
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